This was followed by incubation on ice for 20min and centrifugation at 10,000 × g for 15min. The control sample had the reaction mixture same as above but the enzyme was added after TCA
This was followed by incubation on icefor 20min and centrifugation at 10,000 × g for 15min. Thecontrol sample had the reaction mixture same as above butthe enzyme was added after TCA