methanol production was significantly (p < 0.05) decreased (Fig. 2).
Based on these results, strain SAD2 can be grown on a CH4/air mixture
containing H2S up to1000 ppm, and it can convert methane to
methanol in the presence of H2S up to 500 ppm, which is above the
H2S level in the biogas from most anaerobic digesters. It should be
noted that air needs to be mixed into the biogas to provide O2 for
methanotrophs to respire, resulting in dilution of H2S present in
biogas. Thus, strain SAD2 may be used in microbial conversion of
biogas to methanol.