7. Invert the funnel so that the liquid is not touching the stopcock.
8. Vent the funnel by turning the stopcock such that it is parallel with the outlet of the funnel.
9. Shake the funnel gently. We want to mix the layers, however we don’t want to create an emulsion that will keep us in lab all day.
10. Return the separatory funnel to the ring stand, and remove the stopper. If you forget to remove the stopper, you will be there all day, waiting for the liquid to drain.
11. Drain off and save the appropriate layer(s), and repeat the above process as needed.