Figure 9. Effect of Al (20 mM, 3 h) on the expression of myosin VIII probed with polyclonal
antibody and its colocalization with Al-induced
callose in wheat cv Scout 66 root apex. Control
images (A and A9) show myosin VIII fluorescence associated with inner cortex pit-fields in
the form of fine dots, except bright fluorescence
at callosic cell plates (arrows in A and A9). In
contrast, overall brighter fluorescence is apparent after Al treatments (B, B9, and C). B (longitudinal cell walls, arrows) and B9 (paradermal
section, arrow) images document the Alinduced myosin VIII accumulation at callosic
pit-fields of cortex cells. The Al-induced callose
deposition lining the PM of the outermost cortex
cells facing the epidermis is not associated with
proportional myosin VIII accumulation (compare C and C9 with Fig. 8, C and C9, for calreticulin). All images are from the 1- to 2-mm
region of the root apex. Sections labeled without
the primary antibody showed no fluorescence
signals (D). Representative images of at least
two independent experiments comprising four
to six individual root apices. Scale bar represents 12 mm.
Figure 9. Effect of Al (20 mM, 3 h) on the expression of myosin VIII probed with polyclonal
antibody and its colocalization with Al-induced
callose in wheat cv Scout 66 root apex. Control
images (A and A9) show myosin VIII fluorescence associated with inner cortex pit-fields in
the form of fine dots, except bright fluorescence
at callosic cell plates (arrows in A and A9). In
contrast, overall brighter fluorescence is apparent after Al treatments (B, B9, and C). B (longitudinal cell walls, arrows) and B9 (paradermal
section, arrow) images document the Alinduced myosin VIII accumulation at callosic
pit-fields of cortex cells. The Al-induced callose
deposition lining the PM of the outermost cortex
cells facing the epidermis is not associated with
proportional myosin VIII accumulation (compare C and C9 with Fig. 8, C and C9, for calreticulin). All images are from the 1- to 2-mm
region of the root apex. Sections labeled without
the primary antibody showed no fluorescence
signals (D). Representative images of at least
two independent experiments comprising four
to six individual root apices. Scale bar represents 12 mm.
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