2. Materials and methods
2.1. Diet preparation
Six isonitrogenous (48% crude protein) and isoenergetic (ca. 15 MJ
gross energy kg−1
) semi-purified diets were formulated to contain
graded levels ofwheat starch (uncooked) from5 to 30% (Table 1). Fish
meal, casein and gelatin were used as protein sources, and pollock
liver oil was used as the lipid source. Isoenergetic diets were made by
adjusting the lipid and cellulose content. Diet ingredients wereground through an 80-mesh screen. Vitamins and minerals were
mixed by the progressive enlargement method (Zhou et al., 2007a).
Lipid and distilled water (40%, w/w) were added to the premixed dry
ingredients and thoroughlymixed until homogenous in a Hobart-type
mixer. The 1-mm diameter pellets were wet-extruded, and then air-
dried, sealed in plastic bags and stored frozen at −20 °C until used.
2. Materials and methods2.1. Diet preparationSix isonitrogenous (48% crude protein) and isoenergetic (ca. 15 MJgross energy kg−1) semi-purified diets were formulated to containgraded levels ofwheat starch (uncooked) from5 to 30% (Table 1). Fishmeal, casein and gelatin were used as protein sources, and pollockliver oil was used as the lipid source. Isoenergetic diets were made byadjusting the lipid and cellulose content. Diet ingredients wereground through an 80-mesh screen. Vitamins and minerals weremixed by the progressive enlargement method (Zhou et al., 2007a).Lipid and distilled water (40%, w/w) were added to the premixed dryingredients and thoroughlymixed until homogenous in a Hobart-typemixer. The 1-mm diameter pellets were wet-extruded, and then air-dried, sealed in plastic bags and stored frozen at −20 °C until used.
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