At 48 h p.m., raw meat samples from the LL muscle, cut into 2.5 cm
thick slices and placed in an oven bag (a plastic bag used for cooking
meat), were heated in water at 80–81 °C until the internal temperature
of themeat samples reached 72 °C, and were then subjected to sensory
evaluation after cooling to 20 °C. Samples had approximately equal sizes
(about 25 g) andwere placed in lidded one ounce glass jars labeledwith
three digit randomcodes and held in awater bath (54 °C) until presented
to the panelists. The analysis was conducted in rooms at daily light
and at room temperature (20 °C). To neutralize the taste, each person
received hot tea without sugar between the assessments of samples.
Sensory evaluation of the LL samples was performed to determine
their color, aroma, tenderness, juiciness and flavor. This evaluation
was performed in 12 sessions by a team of 4 to 5 trained individuals
using a 5-point scale (1 = unacceptable and 5 = very acceptable) according
to PN-ISO 4121:1998. At each session, the panelists evaluated
10 randomly presented samples which were served at the same time
to each of the panelists. Two sessions were held each day.