Extraction method
The supercritical extraction was accomplished in the Department
of Chemical Sciences at the University of Monserrato
(Cagliari, Italy). Supercritical CO2 extractions were performed
in a laboratory apparatus equipped with a 400 cc extraction
vessel, which operated in a single-pass mode by passing CO2
through the fixed bed of vegetable materials. Two fractions
of the extract were recovered in two separator vessels, connected
in a series (300 and 200 cc). The cooling of the first separator
was achieved by using a thermostatic bath (Neslab,
Model CC-100II, accuracy of 0.1 C). The use of the second