The concentrations of ALA, EPA, DHA, and total ao 3 fatty cids in the yolk in this study were lower compared to previously reported findings(Aymond and Van Elswyk 1995; Ferrier and and 1995; and others 1995, Coates 1999; Be and The substantial differ ences could be due to several reasons.
The fish oil used in this study was commercial feed grade fish oil and not pure menhaden fish oil, which may have a lower EPA and DHA levels.
Second, previous studies by Ayerza and Coates(2000), Ferrier and others(1995), and Van Elswyk(1997b) used different breeds of hens and this may have influenced the result. Furthermore, the feeding trial by Ayerza and Coates(2000) was conducted over a longer period(90 d period) of time and Bean and Leeson(2003) implemented a"phase in period" in their study to gradually introduce the diet treatments.
In addition, the incorporation of ALA and DHA into egg yolk is a gradual process and may take up to several weeks
The concentrations of ALA, EPA, DHA, and total ao 3 fatty cids in the yolk in this study were lower compared to previously reported findings(Aymond and Van Elswyk 1995; Ferrier and and 1995; and others 1995, Coates 1999; Be and The substantial differ ences could be due to several reasons. The fish oil used in this study was commercial feed grade fish oil and not pure menhaden fish oil, which may have a lower EPA and DHA levels. Second, previous studies by Ayerza and Coates(2000), Ferrier and others(1995), and Van Elswyk(1997b) used different breeds of hens and this may have influenced the result. Furthermore, the feeding trial by Ayerza and Coates(2000) was conducted over a longer period(90 d period) of time and Bean and Leeson(2003) implemented a"phase in period" in their study to gradually introduce the diet treatments. In addition, the incorporation of ALA and DHA into egg yolk is a gradual process and may take up to several weeks
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