then there was a confirmative test
for thermo tolerant coliforms, with the transfer of an extract to tubes
with a Brilliant Green-Lactose-Bile Broth at 2% and incubated at 36 ±
2ºC for 48 h. To confirm the presence of thermo tolerant coliforms,
some of the substance in the positive tubes was transferred to LSB and
inoculated in tubes with E. coli broth (EC)