Microscopic study. Under local anesthesia with lidocaine hydrochloride 1%, tissue specimens (0.5 cm × 0.5 cm) were taken from right and left horn buds, 5 and 10 days after injection of clove essence in groups 2 and 3, respectively. The samples from the skin bud and underlying tissue were fixed in 10% buffered formalin. The samples were embedded in paraffin, sectioned at 4 μm, and stained with hematoxylin and eosin (H & E) for light microscopy.