50μg/ml of stock solution was prepared by
dissolving 5mg of Ciprofloxacin in 100ml of
phosphate buffer having pH of 2.5 and pH 7.4
respectively , in 0.1N HCl and in Distilled water.
Standard solution of various concentrations (1,
2, 3, 4, 5μg/ml) were prepared by dilution from
each stock solution. The wavelength of
maximum emission (λmax) of Ciprofloxacin in
each media was found by scanning them over
the UV range of 190nm to 400nm. Concentration
vs. absorbance was plotted and standard curve
was prepared by scanning the standard
solutions of Ciprofloxacin at 278nm in the media
of Buffer pH 2.5, at 270nm in the media of Buffer
pH 7.4, at 277nm in the media of 0.1N HCl, at
278nm in the media of Distilled water, presented
in Figure 2.