non-dermatophytic organism was Aspergillus Niger spp. (10.00%). Nested PCR was positive in 52
(65.00%) of nail samples while real time PCR was positive in 58 (72.5%) of nail samples.
Conclusion: Real-time PCR followed by melting-point analysis, gives a diagnostic tool that has a
higher sensitivity (93.3%) and is faster than nested PCR (73.3%) and other conventional methods.
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