The preliminary Kombucha SCOBY was purchased from Dina Farms in Egypt. While Lipton black tea (100 tea bags, a total of 226 g) was purchased from the local grocery store. On the other side, Carboxymethylcellulose was purchased from Sigma Aldrich. For antimicrobial tests, microorganisms of C. albicans ATCC 10231, Salmonella sp. ATCC 14028, E. coli ATCC 8739, P. aeruginosa ATCC 9027, and S. aureus ATCC 6538 were supplied by TCS Biosciences Ltd., United Kingdom. L. monocytogenes ATCC 7646 and E.