The MTT Cell Proliferation Assay measures the cell proliferation rate and conversely,
when metabolic events lead to apoptosis or necrosis, the reduction in
cell viability. The number of assay steps has been minimized as much as possible
to expedite sample processing. The MTT Reagent yields low background
absorbance values in the absence of cells. For each cell type the linear relationship
between cell number and signal produced is established, thus allowing an
accurate quantification of changes in the rate of cell proliferation.