Shoot elongation
The shoots from the most appropriate media were dissected from
primary explants and subcultured for further growth in shoot elon-
gation media. Based on the results of preliminary experiments, shoot
elongation media contained MT basal media with 30 gr L-1 sucrose
and BA as cytokinin source at two concentrations (0.2 and 0.5 mg L-1)
along with four concentrations of gibberellic acid (GA3) (0, 0.1, 0.2
and 0.5 mg L-1). Shoots were cultured and maintained at the same
growth conditions mentioned for shoot induction, and were subcul-
tured to rooting media after four weeks.