Hydrothermal treatment
Hydrothermal process was performed according to the
method described by Mosharraf et al. (2009) with slight
modification. Bran of different particle sizes (300 g) was
wet-steeped in 2 volumes of acetate buffer (pH 4.8) at 55 °C for 60 min during which the buffer was replaced by new
buffer solution twice. Afterwards the bran was incubated at
55 °C for 24 h and then it was washed several times with
distilled water to reach the initial pH (6.20±0.2) and dried in
an oven at 50 °C to reach moisture content of 10.9±0.03 %.