TSB-YE, NI-IgY (400 mg mL−1 in TSB-YE)
and the commercial antibiotic ciprofloxacin (10 μgmL−1 in TSBYE)
were used as blank, negative andpositive controls respectively.
The prepared solutions were centrifuged at 12 000 × g for 30 min
and the supernatants were collected and sterilised by filtration
through a microfilter (0.22 μm pore size).