2.3. Fungicide treatments and sampling
Seven experimental plots of 225 m2 were selected (one control
and six for the individual treatments with the fungicides under
study). All treatments were performed under critical agricultural
practices (CAP), i.e., six hours before grape collection. Despite this,
the final results in wines for the commercially products used were
famoxadone 0.12 mg/l, trifloxystrobin < 0.05 mg/l; fluquinconazole
< 0.05 mg/l, quinoxyfen < 0.01 mg/l; fenhexamid 0.89 mg/l,
and kresoxim-methyl 0.08 mg/l, all of these under their MRL
(Table 1). Table 1 also shows the application doses. The experimental
plots had not received previous treatment with the fungicides
studied and at the time of application, grapes were exempt from
any pesticide residues.
Grape samples (15 kg) were destemmed and crushed, and then
80 mg/l of SO2 was added. Musts were inoculated with active dry
yeast UCLM S377 (Anfiquimica SL) following the protocol of the
manufacturer. Samples were introduced in the fermenters and
maintained in dynamic maceration for 8 days at controlled