Extractives present in biomass comprised of non-structural
aromatic compounds, which possess one or more phenolic
hydroxyl groups [26]. These components are different types of fatty acids, resins, tannins etc; all these materials have
a prominent effect on analysis of structural carbohydrates
and lignins. The knowledge of the amount of extractives helps
to estimate exact amount of fermentable sugars present in
biomass. For estimation of extractives in the biomass samples
NREL protocol was use