the PCR protocol included an initial denaturation at 95 C for 5 min,
followed by 25 cycles of 95 C for 30 s, 56 C for 30 s, and 72 C for
90 s, and a final extension at 72 C for 10 min. Amplicons were
analyzed by 1% agarose gel electrophoresis with Goldview staining,
and photographed using a UV Imaging System