Multiple 5 m unstained tissue sections
were mounted on glass slides. To specifically extract
the fetal chorionic villi from the maternal
decidual tissue, the PixCell II (Arcturus, Carlsbad,
CA, USA) LCM system was applied according to
the manufacturer’s specifications, as previously
described (7-9). In brief, the tissue sample
mounted on a glass slide was first studied under a
light microscope.