Among organic nitrogen sources tested, a mixture of yeast extract and peptone (1:1) gave the
best biomass (17.05 g/L for T. spathulata and 11.1 g/L for K. ohmeri) and the maximum lipid production (10.43 g/L
for T. spathulata and 4.53 g/L for K. ohmeri). In the view point of economic strategy, the cheaper inorganic nitrogen
sources were also tested.