Similar results were observed by Lee et al. (2013) who testing the strain S. cerevisiae NK28 at different initial concentrations of sucrose, obtained a maximum ethanol concentration of 85.56 g/L with 200 g/L sucrose, but when the sugar concentration increased to 300 and 400 g/L, ethanol concentration decreased considerably to 46.58 and 1.11 g/L respectively. In our case, although S. cerevisiae strains A2, A10, and A11 tolerated up to 300 g/L without decreasing
its production of ethanol, it can be deduced from the above results that the concentration that ensures high concentrations of alcohol is 250 g/L of sugar.