Infectious kidney and spleen necrosis virus (ISKNV) is the type species of the genus Megalocytivirus of the family Iridoviridae .The genome of ISKNV is a single dsDNA with the length of 111,362 bp, which encodes 125 potential open reading frames
(ORFs) . ISKNV is the causative agent of Mandarin fish (Siniperca chuatsi) infectious spleen and kidney necrosis (ISKN) and has caused high mortality and great economic losses in the culture of Mandarin fish in Asian countries. Clinical signs of ISKN include anorexia, abnormal swimming, darkened color, ulcerative gills, red
spotted livers, swollen spleens and kidneys. Identification of host factors in response to ISKNV infection has a great significance for the prevention of ISKN in Mandarin fish culture. There have been several studies concerning the ISKNV infection have been reported [3e9]. However, the molecular mechanisms that underlie the pathogenesis of the infection remain poorly understood