Fig. 7(a-f): Shoot regeneration from callus (a-b) Shoot initiation from white and friable callus, (c-d) Developing of shoots from callus and (e-f) Developed shoots for transfer into rooting medium
Yildizi et al. (2007) has been reported that sucrose can cause the tissue blackening and browning by the oxidation of phenolic compounds which are exuded from cut surfaces of explants has caused serious problems in establishing in vitro culture and the exudates inhibit growth and tissue necrosis. In this experiment, sucrose concentration was decreased from 3 to 1-2% and necrosis was significantly decreased in tissue samples. Figure 8 shows the necrosis tissues caused by sucrose phenolic compound effect.