Table 7
Frequency scores (%) for the fivemorphological parameters used to assess the presence of enteritis in giant grouper fed the experimental diets. Scoreswere based on observations of distal
intestines from three fish per treatment (one fish per tank) using a semi-quantitative scale of 1–5 (with 5 being the most overt sign of enteritis).
Parameter Diet
FM100 FM80 FM60 FM20
1 2 3 4 5 1 2 3 4 5 1 2 3 4 5 1 2 3 4 5
Shortening of mucosal folds (MF) 100 – – – – – 100 – – – – 33 67 – – – – – – 100
Number/size of goblet cells (GC) 100 – – – – – 100 – – – – – 33 67 – – – – – 100
Width of lamina propria (LP) 100 – – – – – 67 33 – – – – – 100 – – – – 33 67
Presence of supranuclear vacuoles (SNV) 100 – – – – 67 33 – – – – – 33 67 – – – – – 100
Thickness of sub-epithelial mucosa (SM) 100 – – – – 100 – – – – 100 – – – – – 100 – – –