The SHF experiments started with the inoculation of pineapple peel with the addition of the
enzyme solution (Cellulase, 100 FPU/mL) in 50 mL working volume for 0-96 h. Therefore, a filtersteriled
hydrolysate produce from pineapple peel under optimal condition was used as fermentation
medium with no nutrient supplementation. Afterward free and immobilized cell of S. cerevisiae and E.
aerogenes with a loading of 20% (w/v) was operated under anaerobic condition and a temperature
controlled at 30oC.