Rainbow trout “eyed ova” from a regional trout hatchery were
placed in an aerated, re-circulating laboratory aquarium, maintained
at 10–15 C with a biological
filter to detoxify faecal
ammonia. Lipid was extracted from an additional 2050 rainbow
trout eggs to determine their vitamin D content by a chick toe–ash
bioassay [8]. The hatched
fish were reared on a semi-purified,
vitamin D-free diet, prepared in the form of soft gelatin pellets
(Table 1). The trout were kept in total darkness except for 10 min
each day, during feeding, when the aquarium room was lit with a
dim 60 W incandescent ceiling light with a minimum wavelength
emission of about 400 nm.
Rainbow trout “eyed ova” from a regional trout hatchery wereplaced in an aerated, re-circulating laboratory aquarium, maintainedat 10–15 C with a biologicalfilter to detoxify faecalammonia. Lipid was extracted from an additional 2050 rainbowtrout eggs to determine their vitamin D content by a chick toe–ashbioassay [8]. The hatchedfish were reared on a semi-purified,vitamin D-free diet, prepared in the form of soft gelatin pellets(Table 1). The trout were kept in total darkness except for 10 mineach day, during feeding, when the aquarium room was lit with adim 60 W incandescent ceiling light with a minimum wavelengthemission of about 400 nm.
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