The thermal cycling process and fluorescence signal detection were
carried out with the Applied Biosystems 7500 Real-Time PCR System (ABI, Foster City, CA, USA).
The cycling parameters were: reverse transcription for 5 min at 42 °C, denaturation for 10 s at 95 °C,
followed by 40 cycles of 10 s at 95 °C for denaturation and 36 s at 62 °C for annealing and extension