Preparation of bone ash: Tibial bones of the cattle
were collected from a nearby slaughter house. The bones were cut into 2 × 2 pieces using a prebeaker. Then the bone
pieces were incinerated at 300◦C and ashed at 750◦C for
about 5 h. This treatment made organic matter present in the
bone to get volatilized and resulted in the formation of bone
ash, which was nothing but the inorganic part of the bone
which was denoted as bone ash (BA).