chick embryo was harvested. Brain was collected on ice, washed with ice-cold buffer (25 mM Tris- HCl, 1 mM EDTA, pH 7.5), homogenized and stored at −80oC.
chick embryo was harvested. Brain wascollected on ice, washed with ice-cold buffer (25 mM Tris-HCl, 1 mM EDTA, pH 7.5), homogenized and stored at−80oC.