The Melle-Boinot process is the typical process for fuel
ethanol production by batch fermentation. This process
comprises the weight and sterilization of feedstock, followed
by the adjustment of pH with H2SO4 and of the
degrees Brix to values of 14–22. Obtained wort is fermented
by yeasts. The produced wine is decanted, centrifuged and
sent to ethanol separation stage, whereas the yeasts are
recycled to the fermentation in order to reach high cell concentration
during cultivation (Kosaric and Velikonja,
1995).