The culture of C. albican was kept on SDA at 4°C. Cul-ture from plate was inoculated in YEPD broth and incu-bated at 37°C for 24 hrs. Yeast cells were washed twice by centrifugation with sterile water and resuspended in water. The turbidity of suspension was adjusted to 0.5 Mc Farland solutions (1 × 106 to 5 × 106 CFU/ml).