Previous studies, reported about relationships of many beetles and Scarabaeinae by using several molecular markers to examine evolution and phylogeny (Hunt et al., 2007 and Monaghan et al., 2007). The current studies about phylogenetic relationships of Dung beetles by using marker of molecular phylogeny were mitochondrial DNA (mtDNA) and nuclear DNA. Mitochondrial DNA (mtDNA) is highly conserved, maternal inheritance (Avise, 1995), highly rate of mutation, variation in mitochondrial depend on mutation, can be testing population monophyly and then, mtDNA is used in evolutionary studies. Furthermore, nuclear DNA is important. Nuclear DNA gene regions which are powerful for genealogical and evolutionary studies of populations.