(2 to 3 cm in length) were washed with
0.1 M phosphate buffered saline (pH 7.4), fixed in Bouin’s
solution for 6 h and dehydrated in a graded ethanol
(xylene) series. Each segment was embedded in paraffin
wax using standard technique. The values of villus area
were calculated from the villus height, basal width and
apical width according to the method described by