Supporting parameters and statistical analysis
Fish sample pH was measured from TPC initial dilution (see section 2.4) using pH meter (EZDO pH 5011).
Water content was analyzed using the method described in SNI 01-2354.2-2006. Briefly, drying oven was set up for
heating at 105 °C. Empty plates were kept in the oven for 1 h minimum at the same temperature. The preheated
empty plates were then displaced into a desiccator for about 30 min and weighed. Pounded fish samples (2 g) were
placed into the conditioned plates and dried at the oven for 16 h to 24 h at 105 °C. The plates were removed from the
oven into a desiccator after the drying period was over, and kept there for another 30 min and then weighed. The
water content was calculated using the following equation,