Further processing
was done according to the instruction of Ultra Vision plus detection
system, anti-polyvalent, HRP/DAB (Ready-to use) staining kit
(Thermo scientific system). The peroxide complex was visualized
with 3,30-diaminobenzidine (DAB). Lastly the slides were counterstained
with haematoxylin and dried. Finally the sections were
mounted with DPX and covered with cover slips. The slides were
ready to be observed under microscope. For quantitative immunohistochemical
evaluation,