The pellet was dispersed in 50 μL of Dulbecco's Phosphate-Buffered Saline
(PBS; pH 7.3; Oxoid) by vortexing and the cell suspension added
to 15 mL of the OJ sample in a 25 mL universal bottle to achieve a final
concentration of 2 × 108 cells/mL. Cells were dispersed in the OJ by
vortexing the bottle for 12 s.