STZ-induced diabetic rats were also
divided into 3 groups with 4 animals in each group
in the same pattern as described for normal rats.
After 6 weeks of treatments, both normal rats and
STZ-induced diabetic rats were fasted for 12 hours
and then the basal and the glucose stimulated
insulin secretion were examined. The tail vein
blood was collected at before glucose loading for
measuring basal insulin level and at 30 and 60 min
after glucose loading (2 g/kg, orally) for measuring
glucose stimulated insulin secretions.