Cell suspension of alga was periodically collected from the reactor after second transfer to the fresh media. The sample was determined the OD435 and cell dry weight; and for extraction of total
hydrocarbon. Percentage of cell growth was calculated from [(OD435,t -ODm.initial) x 1001 OD435.initial], where OD435.initial and OD4)5.t were ODm at the beginning (t=0) and at time t, respectively.