The fractions were concentrated under reduced pressure at 35 or 65 C to remove solvents. The yields of ethyl ether, ethyl acetate, butanol and aqueous fractions were 0.9, 5.5, 7.3
and 7.4 g, respectively. The total phenolic content (TPC) in each fracton was determined by the Folin–Ciocalteu method . One milliliter of extract in methanol was mixed with 1 ml of 3-fold-diluted Folin–Ciocalteu phenol reagent. Two milliliters of 35% sodium carbonate solution and distilled water were added subsequently. The mixture was incubated for 30 min and the absorbance was recorded at 700 nm by a spectrophotometer