The homogenate was stirred at 4 C for 1 h. After centrifugation for 20 min at 12 500 rpm, the supernatant was filtered through Whatman No. 4 paper, and used immediately for the assays.
The homogenate was stirredat 4 C for 1 h. After centrifugation for 20 min at 12500 rpm, the supernatant was filtered through WhatmanNo. 4 paper, and used immediately for the assays.