Grasshoppers were
individually marked with acrylic colours (Lukas CRYL TERTIA).
Experiments tookplace inacagewithabasearea of12.5 cm 9.5 cm
and a height of 5.5 cm. The cagewas heated to constant 30 8Cwitha
heat lamp and additionally illuminated with two light bulbs. For
analysis, videos were later reviewed on a monitor (HR Trinitron,
SONY) allowing the identification of previously described components
of reproductive behaviour. We evaluated the occurrence of
male courtships, female stridulation, female rejections or failures of
male mounting attempts and successful mountings of females by
males. Mountings were counted as successfulmatings if they lasted
longer than 2min. The five groups of females were tested on 13
consecutive days resulting in a total of 65 observation periods. On a
single experimental day, the different groups of females encountered
differentmales thathadbeenisolatedfromfemales for at least3days.
All experimental datawere analysedwith Excel (Microsoft) and SPSS
PASW Statistics 18.0 software (SPSS Inc., Chicago, US).
Grasshoppers wereindividually marked with acrylic colours (Lukas CRYL TERTIA).Experiments tookplace inacagewithabasearea of12.5 cm 9.5 cmand a height of 5.5 cm. The cagewas heated to constant 30 8Cwithaheat lamp and additionally illuminated with two light bulbs. Foranalysis, videos were later reviewed on a monitor (HR Trinitron,SONY) allowing the identification of previously described componentsof reproductive behaviour. We evaluated the occurrence ofmale courtships, female stridulation, female rejections or failures ofmale mounting attempts and successful mountings of females bymales. Mountings were counted as successfulmatings if they lastedlonger than 2min. The five groups of females were tested on 13consecutive days resulting in a total of 65 observation periods. On asingle experimental day, the different groups of females encountereddifferentmales thathadbeenisolatedfromfemales for at least3days.All experimental datawere analysedwith Excel (Microsoft) and SPSSPASW Statistics 18.0 software (SPSS Inc., Chicago, US).
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