Tocopherols and tocotrienols were first extracted with xylene, and the absorbance was read at 460 nm. Then, after adding ferric chloride, absorbance was read at 520 nm using spectrophotometer (Systronics UV–VIS-108, Bangalore, India).
Tocopherols and tocotrienols were first extracted withxylene, and the absorbance was read at 460 nm. Then, after addingferric chloride, absorbance was read at 520 nm using spectrophotometer(Systronics UV–VIS-108, Bangalore, India).