The fermentation of acid and enzymatic hydrolysates was
carried out separately in 1.0 L Erlenmeyer flasks, each containing
250 mL of hydrolysate. The acid hydrolysate
(38.43 g L1 sugars) supplemented with (g L1
) NH4Cl, 0.5;
KH2PO4, 2.0; MgSO4$7H2O, 0.5; yeast extract, 1.5; CaCl2$2H2O,
0.1; FeCl3$2H2O, 0.1; ZnSO4$7H2O, 0.001 (pH 5.5 0.2),
was inoculated with 8.0% inoculum of P. stipitis NCIM 3499
(OD600 0.6) and incubated at 30 C for 36 h under shaking
conditions (2.5 Hz). The enzymatic hydrolysate (36.47 g L1
sugars, pH 6.0 0.2) added with 3 g L1 yeast extract and
0.25 g L1 (NH4)2HPO4 was inoculated with S. cerevisiae
HAU (6.0%) and incubated at 35 C and 3.33 Hz. Samples
were withdrawn at regular intervals of 4 h and centrifuged at
Tab
The fermentation of acid and enzymatic hydrolysates wascarried out separately in 1.0 L Erlenmeyer flasks, each containing250 mL of hydrolysate. The acid hydrolysate(38.43 g L1 sugars) supplemented with (g L1) NH4Cl, 0.5;KH2PO4, 2.0; MgSO4$7H2O, 0.5; yeast extract, 1.5; CaCl2$2H2O,0.1; FeCl3$2H2O, 0.1; ZnSO4$7H2O, 0.001 (pH 5.5 0.2),was inoculated with 8.0% inoculum of P. stipitis NCIM 3499(OD600 0.6) and incubated at 30 C for 36 h under shakingconditions (2.5 Hz). The enzymatic hydrolysate (36.47 g L1sugars, pH 6.0 0.2) added with 3 g L1 yeast extract and0.25 g L1 (NH4)2HPO4 was inoculated with S. cerevisiaeHAU (6.0%) and incubated at 35 C and 3.33 Hz. Sampleswere withdrawn at regular intervals of 4 h and centrifuged atTab
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