These protocols have been used successfully for bacteria such as Escherichia coli,
Bacillus subtilis, Bordetella pertussis from nasopharyngeal swabs, Borrelia burgdorferi
from cerebrospinal fluid, and Legionella pneumophila from broncho-alveolar lavage.
For other bacteria, follow the protocol for Gram-positive bacteria, especially other
Gram-positive bacteria, which may be difficult to lyse