Another assessment of detection sensitivity in relation to smear positivity and CFU was performed with 24 seeded specimens. Cell concentrations equivalent to 103, 10 4, and 105 dilutions of a 0.5 McFarlandstandard yielded smear results of 1,doubtful/borderline (1 to 3 AFB per 100 fields at 400 magnification), and negative, respectively. The specimens with positive smear results yielded 100% sensitivity and correct sequences in each subassay. The smear-negative specimens yielded 80 to 240 CFU/ml and 100% sensitivity (8/8 specimens) in the IS6110, inhA, and rpoB subasay
Another assessment of detection sensitivity in relation to smear positivity and CFU was performed with 24 seeded specimens. Cell concentrations equivalent to 103, 10 4, and 105 dilutions of a 0.5 McFarlandstandard yielded smear results of 1,doubtful/borderline (1 to 3 AFB per 100 fields at 400 magnification), and negative, respectively. The specimens with positive smear results yielded 100% sensitivity and correct sequences in each subassay. The smear-negative specimens yielded 80 to 240 CFU/ml and 100% sensitivity (8/8 specimens) in the IS6110, inhA, and rpoB subasay
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