Of the five, line nh136 was
most identical to XB(GG) except for panicle size and
was designated as XB(TT) (Lane 7 in Fig. 1). Meanwhile,
the TT Wx genotype was transferred from
XB(TT) into the isogenic male sterile line XA(TT) by
crossing and recurrent backcrossing with XA(GG)
(Lane 8 in Fig. 1). The AC of the two improved lines,
057 (TT) and XB(TT), were reduced to 14.3 and
11.9% from 27.3 to 25.0%, respectively. The GBSS
activities dropped to 5.8 and 5.0 nmol/g min from
10.2 to 9.4 nmol/g min, respectively. The AC of
XA(TT) was also reduced from 25.2 to 10.7%
(Table 2).