The research done on ABE as an alternative fuel in engines is still at an early stage. To date, the use of water-containing ABE– gasoline blends in SI engines has not been investigated. Therefore, the objective of this study is to evaluate the use of watercontaining ABE–gasoline blends in a port fuel-injected SI engine based on performance, combustion and emissions characteristics of various fuels, including pure gasoline (G100), ABE30, ABE85, ABE29.5W0.5 and ABE29W1 (29 vol.% ABE, 1 vol.% water and 70 vol.% gasoline).