Epoxy functionalized silica (1 g) was mixed with 10mg lipase
in 10 ml phosphate buffer 25mM (pH 7) followed by incubation
at 25 ◦C for 24 h. Immobilized lipase was recovered by filtration,
washed thoroughly with distilled water, and then dried overnight
at room temperature.
3-Glycidyloxypropyl trimethoxysilane (3-GPTMS) was purchased
from Acros. All other chemicals were obtained commercially and
were of analytical reagent grade.